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Image Search Results
Journal: World Journal of Stem Cells
Article Title: Patient-derived induced pluripotent stem cells with a MERTK mutation exhibit cell junction abnormalities and aberrant cellular differentiation potential
doi: 10.4252/wjsc.v16.i5.512
Figure Lengend Snippet: Generation and identification of the human induced pluripotent stem cells derived from the retinitis pigmentosa patient. A: Timeline of human induced pluripotent stem cell generation; B: Imaging by phase-contrast microscopy. Scale bar = 200 μm; C: Karyotype analysis of the healthy control (left) and retinitis pigmentosa patient (right); D: Flow cytometry of pluripotency markers SSEA-4 and TRA-1-81; E and F: Immunostaining of pluripotency markers OCT4, NANOG, SOX2, and SSEA4 of the healthy control and retinitis pigmentosa patient. Scale bar = 20 μm; G and H: In vitro differentiation of control (G) and patient (H) induced pluripotent stem cells into three germ layers, endoderm (AFP+), mesoderm (α-SMA+) and ectoderm (GFAP+). Scale bar = 20 μm. PB: Peripheral blood; PBMC: Peripheral blood mononuclear cell; iPSC: Induced pluripotent stem cell.
Article Snippet: Following primary antibodies were used: OCT4 (Cat. #ab18976; Abcam), SOX2 (Cat. #sc-365823; Santa Cruz), NANOG (Cat. #ab80892; Abcam), SSEA4 (Cat. #ab16287; Abcam), GFAP (Cat. #HPA056030; Sigma), α-SMA (Cat. #A5228; Sigma),
Techniques: Derivative Assay, Imaging, Microscopy, Control, Flow Cytometry, Immunostaining, In Vitro
Journal: Cell reports
Article Title: Critical roles of translation initiation and RNA uridylation in endogenous retroviral expression and neural differentiation in pluripotent stem cells
doi: 10.1016/j.celrep.2020.107715
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Antibodies and dilution rate were as follows; mouse monoclonal anti-OCT3/4 (1:200, sc-5279, Santa Cruz Biotechnology), rabbit polyclonal anti-SOX2 (1:100, ab97959, Abcam), rabbit polyclonal anti-NANOG (1:100, ab21624, Abcam), mouse monoclonal anti-human Nuclei (1:1000, MAB4383, EMD Millipore), rabbit polyclonal anti-AFP (1:200, A0008, DAKO),
Techniques: Recombinant, Protease Inhibitor, Modification, Knock-Out, Transfection, Blocking Assay, Western Blot, Immunocytochemistry, Lysis, Purification, Clone Assay, SYBR Green Assay, Reporter Assay, TaqMan Assay, Expressing, Microarray, shRNA, Plasmid Preparation, Software